منابع مشابه
Neurospora crassa a mating-type region.
The a mating-type region of Neurospora crassa controls several major events in both the sexual and asexual phases of the fungal life cycle. This 3235-base-pair DNA segment is not homologous to the comparable genetic region of the A mating type. The unique a and A regions are bordered by nearly identical DNA sequences. The a genetic region contains at least two functional segments. One segment e...
متن کاملMutations of the a Mating-Type Gene in NEUROSPORA CRASSA.
In Neurospora, the mating-type locus controls both mating ( A + a is fertile) and heterokaryosis (A + a is incompatible). The two alleles appear stable: no novel fertility reactions have ever been reported, and attempts to separate fertility and heterokaryon incompatibility functions by recombination have been unsuccessful. In the present approach the locus was studied through a mutational anal...
متن کاملHigh throughput mating tests in Neurospora crassa
Mating type tests in Neurospora crassa are an important way to characterize strains. Since most of the knock-out mutants developed as part of the functional genomics program (Colot et al., 2006) have little obvious phenotype we have undertaken to test the mating type of all of the knock-strains that are sent to the FGSC. Our original mating type test protocol is similar to that described by Smi...
متن کاملIsolation of Neurospora crassa A mating type mutants by repeat induced point (RIP) mutation.
In the filamentous fungus, Neurospora crassa, mating type is regulated by a single locus with alternate alleles, termed A and a. The mating type alleles control entry into the sexual cycle, but during vegetative growth they function to elicit heterokaryon incompatibility, such that fusion of A and a hypha results in death of cells along the fusion point. Previous studies have shown that the A a...
متن کاملMolecular characterization of tol, a mediator of mating-type-associated vegetative incompatibility in Neurospora crassa.
The mating-type locus in the haploid filamentous fungus, Neurospora crassa, controls mating and sexual development. The fusion of reproductive structures of opposite mating type, A and a, is required to initiate sexual reproduction. However, the fusion of hyphae of opposite mating type during vegetative growth results in growth inhibition and cell death, a process that is mediated by the tol lo...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1990
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.87.13.4917